Mixed infection of two groups (16SrI & V) of phytoplasmas in a single Jujube tree in China

Symptoms similar to Jujube witches' broom disease were observed on jujube (Zizyphus jujuba) plants in an orchard in Xuchang City, Henan Province, China. Phytoplasmas were detected in one sample from symptomatic plants by nested PCR assay employing 16S rRNA gene primers P1/P7 followed by R16F2n/R16R2. Virtual RFLP analysis of the resulting products (F2nR2 region) shown that total of two different groups (16SrI and 16SrV) phytoplasmas associated with the infected jujube. This is the first report of phytoplasmas mixed infection of jujube in China.