Transcellular transport of aconitine across human intestinal Caco-2 cells.

•The bidirectional transports of aconitine across Caco-2 cells were investigated.•The influx of aconitine was temperature-, pH- and Na+-dependent.•The uptake of aconitine may be mediated by a sodium-dependent glucose transporter.•The efflux of aconitine was temperature- and pH-dependent, but Na+-independent.•The active efflux of aconitine was mediated mainly by P-gp.