AFM and LSCM studies of Choriocarcinoma cell line JAR infected by hepatitis B virus
JOURNAL OF ANIMAL AND VETERINARY ADVANCES(2012)
摘要
Choriocarcinoma cell line JAR was used as a model to study trophoblast. In this study, the morphological changes and three-dimensional reconstruction of JAR infected by Hepatitis B Virus HBV) were detected with the use of Atomic Force Microscope ( AFM) and Laser Scanning Confocal Microscope (LSCM). It was clearly shown that the HBsAg located in the cytoplasm or at the edge of the nucleus of JAR cells infected by HBV, the membrane of JAR cells infected by HBV were depressed, boundaries of cytoplasm and nucleus were unclear. The membrane of JAR were covered by particles and the histogram of particles in the negative group distributed from 400-800 nm in diameter, most of paticles were 800 run while the particles of JAR cells infected by BEV ranged from 200-600 urn, most of paticles were 400 mu. HBV serum can infect JAR cells in vitro, the changes of the morphology of JAR cells infected by HBV were related to the damage of the particles on the membrane of JAR cells. AFM can be useful for studying cell pathology.
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关键词
Choriocarcnoma cell line JAR,hepatitis B virus,atomic force microscope,laser scanning confocal microscope,cell pathology
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