A new triterpenoid saponin from Gymnema sylvestre.

Besides four known compounds, a new triterpenoid saponin was isolated from the stems of Gymnema sylvestre. The structure of the new triterpenoid saponin was established as 3 beta,16 beta,22 alpha-trihydroxy-olean-12-ene 3-O-beta-D-xylopyranosyl-(1 --> 6)-beta-D-glucopyranosyl-(1 --> 6)-beta-D-glucopyranoside (1) on the basis of 1D and 2D NMR techniques, including COSY, HMBC, HMQC, and NOESY correlations. Four known compounds 2, 3, 4, and 5 were identified on the basis of spectroscopic data.