Prostaglandin metabolite induces inhibition of TRPA1 and channel-dependent nociception
Molecular Pain(2012)
Abstract
Background The Transient Receptor Potential (TRP) ion channel TRPA1 is a key player in pain pathways. Irritant chemicals activate ion channel TRPA1 via covalent modification of N-terminal cysteines. We and others have shown that 15-Deoxy-Δ12, 14-prostaglandin J 2 (15d-PGJ 2 ) similarly activates TRPA1 and causes channel-dependent nociception. Paradoxically, 15d-PGJ 2 can also be anti-nociceptive in several pain models. Here we hypothesized that activation and subsequent desensitization of TRPA1 in dorsal root ganglion (DRG) neurons underlies the anti-nociceptive property of 15d-PGJ 2 . To investigate this, we utilized a battery of behavioral assays and intracellular Ca 2+ imaging in DRG neurons to test if pre-treatment with 15d-PGJ 2 inhibited TRPA1 to subsequent stimulation. Results Intraplantar pre-injection of 15d-PGJ 2 , in contrast to mustard oil (AITC), attenuated acute nocifensive responses to subsequent injections of 15d-PGJ 2 and AITC, but not capsaicin (CAP). Intraplantar 15d-PGJ 2 —administered after the induction of inflammation—reduced mechanical hypersensitivity in the Complete Freund’s Adjuvant (CFA) model for up to 2 h post-injection. The 15d-PGJ 2 -mediated reduction in mechanical hypersensitivity is dependent on TRPA1, as this effect was absent in TRPA1 knockout mice. Ca 2+ imaging studies of DRG neurons demonstrated that 15d-PGJ 2 pre-exposure reduced the magnitude and number of neuronal responses to AITC, but not CAP. AITC responses were not reduced when neurons were pre-exposed to 15d-PGJ 2 combined with HC-030031 (TRPA1 antagonist), demonstrating that inhibitory effects of 15d-PGJ 2 depend on TRPA1 activation. Single daily doses of 15d-PGJ 2 , administered during the course of 4 days in the CFA model, effectively reversed mechanical hypersensitivity without apparent tolerance or toxicity. Conclusions Taken together, our data support the hypothesis that 15d-PGJ 2 induces activation followed by persistent inhibition of TRPA1 channels in DRG sensory neurons in vitro and in vivo . Moreover, we demonstrate novel evidence that 15d-PGJ 2 is analgesic in mouse models of pain via a TRPA1-dependent mechanism. Collectively, our studies support that TRPA1 agonists may be useful as pain therapeutics.
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Key words
TRPA1,15d-PGJ(2),Mustard oil,Negative modulation,Mechanical hypersensitivity
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