Introducing chemiluminescence resonance energy transfer into immunoassay in a microfluidic format for an improved assay sensitivity.

We report on a novel strategy to improve microfluidic immunoassay sensitivity by introducing chemiluminescence resonance energy transfer (CRET) into the immunoreactions. The proposed CRET-based immunoassay for estradiol (E2, as a model analyte) is one of the most sensitive immunoassay with a limit of detection at 3.6 x 10(-11) M E2 in a microfluidic format.