Aspergillus niger DLFCC-90 rhamnoside hydrolase, a new type of flavonoid glycoside hydrolase.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY(2012)
Abstract
A novel rutin-alpha-L-rhamnosidase hydrolyzing a-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the alpha-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.
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Key words
flavonoid glycoside hydrolase
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