Biocompatibility of polylactic-co-glycolic acid with bone marrow stromal cells of rhesus monkeys in vitro

Background: Biocompatibility of polylactic-co-glycolic acid (PLGA) with different cells has been reported except its biocompatibility with rhesus monkey bone marrow stromal cells (BMSCs). Objective: To observe the biological characteristics of rhesus monkey BMSCs, and to investigate the biocompatibility of PLAG with rhesus monkey BMSCs. Methods: The second passage BMSCs of rhesus monkeys were co-cultured with PLGA for biocompatibility investigation. Light and electron microscopy revealed that BMSCs adhered to and elongated along the PLGA fiber scaffold. BMSCs cultured alone served as controls. Results and Conclusion: After 4 days of co-culture with PLGA, CD29-positive BMSCs adhered to the surface of PLAG fiber scaffold, forming long cell chains. Some of the attached BMSCs elongated along the scaffold. After 72 hours culture in PLAG extract fluid, neurotrophic factors of BMSCs had no difference from those cultured in plain Iscove's Modified Dulbecco's Medium (P > 0.05). After 7 days, there were no differences in morphology, viability and proliferation of BMSCs cultured in PLGA extract fluid and plain Iscove's Modified Dulbecco's Medium. The results confirmed a high biocompatibility between PLGA materials and BMSCs.