基于韭菜ISSR-PCR扩增技术影响因子分析
Bulletin of Agricultural Science and Technology(2013)
Abstract
通过分析不同浓度的DNA模板、引物、dNTP、MgCI2、Taq酶、温度、时间及循环次数等各因素对扩增效果的影响,总结了韭菜ISSR-PCR扩增效果的影响因子及对策,提出了改进措施,为开展韭菜ISSR-PCR扩增技术研究提供参考。
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