Cold inducible promoter driven Cre-lox system proved to be highly efficient for marker gene excision in transgenic barley.

Csaba Éva,Flóra Téglás,Helga Zelenyánszki,Cecília Tamás,Angéla Juhász,Klára Mészáros,László Tamás

Journal of Biotechnology（2018）

引用 14|浏览3

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摘要

•A cold inducible Cre recombination system has been established.•High efficiency marker gene removal was achieved.•The wcs120 promoter proved to be reliable for the purpose.•Thermal separation of Cre transcription from enzyme activity was the most effective.

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关键词

Auto-excision strategy,Site-specific recombination,Transgenic plants,Cold induction

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