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三叶青的高效液相色谱指纹图谱研究

程林,胡轶娟,浦锦宝,梁卫青,郑军献,魏克民

Zhejiang Journal of Traditional Chinese Medicine（2013）

Cited 1|Views24
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Abstract
目的:应用RP-HPLC建立三叶青的色谱指纹图谱。方法:色谱柱:YMC-Pack ODS-A柱(C18,250×4.6mm,5μm);流动相A:甲醇;流动相B:水;流速:1.0ml/min;检测波长:300nm;柱温:25℃;记录时间:112min。结果:获得了该药材的HPLC色谱指纹图谱,含有共有峰7个。结论:本方法样品处理简便,重复性好,可为三叶青的质量控制提供参考。
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