青萝卜花药培养体系的研究

Shandong Agricultural Sciences(2010)

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Abstract
本研究以潍县青和喜诺晓萝卜为试材,针对影响花药培养的因素——活性炭和NO3-/NH4+,以改良Keller培养基为基本培养基进行试验。结果表明,潍县青适宜诱导培养基为添加0.15%活性炭的基本培养基,喜诺晓萝卜的适宜诱导培养基为NO3-/NH4+为2∶1的基本培养基。并据此建立了两种青萝卜的花药培养体系,扩大了可以应用花药培养技术的萝卜基因型范围。
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