HPLC法检测饲料中林可霉素的含量

Jiangxi Feed(2007)

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Abstract
利用HPLC法检测饲料中林可霉素的含量。采用C18色谱柱(150mm×4.6mm,5μm),以乙腈-水-三乙胺(10∶90∶0.3,磷酸调pH值至3.5)为流动相,流速为1mL·min-1,波长214nm。线性回归方程为Y=1.8055X+6.2055(R2=0.9994),林可霉素在浓度为50~500μg/mL范围内的峰面积与浓度呈良好的线性关系。平均回收率99.27%,RSD%为0.60。
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