高效液相色谱法测定降糖化淤胶囊中丹酚酸B的含量
Chinese Journal of Hospital Pharmacy(2010)
Abstract
目的:建立高效液相色谱法(HPLC)测定降糖化淤胶囊中丹酚酸B含量的方法。方法:采用依利特C18色谱柱(4.6mm×250mm,5μm),以甲醇-乙腈-甲酸-水(30:10:1:59)为流动相,流速1.0mL·min-1,检测波长286nm。结果:丹酚酸B对照品线性范围为1.58~11.85μg,r=0.9998,平均回收率为99.33%,RSD为0.54%(n=6)。结论:该方法准确、可靠,可用于本制剂中丹酚酸B的含量测定。
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