高效液相色谱法同时测定多维生素糖浆中5种维生素和山梨酸含量
China Pharmaceuticals(2011)
Abstract
目的建立可同时测定多维生素糖浆中抗坏血酸(VitC)、烟酰胺(VitPP)、盐酸硫胺(VitB1)、核黄素-5'-磷酸钠、盐酸吡多辛(VitB6)及防腐剂山梨酸含量的高效液相色谱法。方法采用高效液相色谱-紫外分光光度法,使用Allthna C18柱(250 mm×4.6 mm,5μm),流动相A为0.01 mol/L己烷磺酸钠溶液(1 000 mL溶液加入三乙胺0.25 mL和醋酸9.5 mL),流动相B为甲醇(梯度洗脱),流速为1.0 mL/min,检测波长为280 nm。结果 VitC,VitPP,VitB1、核黄素-5'-磷酸钠、VitB6、山梨酸的质量浓度分别在0.15~2.0 g/L,0.1~1.0 g/L,0.02~0.4 g/L,0.02~0.4 g/L,0.007~0.1 g/L,0.03~0.6 g/L范围内与峰面积线性关系良好;平均回收率分别为99.9%,98.4%,99.4%,100.9%,100.4%,100.3%,RSD=0.5%,0.6%,0.4%,0.9%,1.0%,0.8%(n=9)。结论该方法快速、灵敏,色谱峰分离度良好,结果准确可靠,可作为多维生素糖浆的质量控制项目之一。
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Key words
ascorbic acid,thiamine hydrochloride,nicotinamide,pyridoxine hydrochloride,HPLC-UV,sorbic acid,riboflavin-5′-sodium phosphate
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