Synthesis of adenine and guanine nucleotides at the 'inosinic branch point' in lymphocytes of leukemia patients.

The synthesis of purine nucleotides has been studied in human peripheral blood lymphocytes from healthy subjects and patients affected by B-cell chronic lymphocytic leukemia (B-CLL). The rate of the synthesis was measured by following the incorporation of C-14-formate into the nucleotides of lymphocyte suspensions. The whole sequence AMP --> ADP --> ATP was found reduced in B-CLL lymphocytes; in the case of guanylates only the last step of the sequence GMP --> GDP --> GTP was significantly lower in the same cells. From the analysis of these results, combined with previous data, we conclude that purine metabolism undergoes an imbalancement during CLL, which is partially compensated, and point out the importance of studying concomitantly purine metabolism and nucleic acid synthesis in leukemia cells. (C) 1999 Elsevier Science B.V. All rights reserved.