毛蚶多糖的分离纯化和免疫活性测定
Chinese Journal of Marine Drugs(2007)
Abstract
目的从毛蚶体内分离纯化得到毛蚶多糖精品(polysaccharide from Arca subcrenata Lischke.简称ASLP),分析其纯度和单糖组成,同时考察免疫活性。方法经除蛋白、DEAE-52和sephadex-G50柱层析得多糖精品。糖醇乙酸酯法测定单糖组成。体外脾淋巴细胞增殖测定ASLP的免疫活性。结果从毛蚶体内提取到一种均一的多糖组分,其单糖组成只有葡萄糖,ASLP能够显著地促进脾淋巴细胞的增殖。结论从毛蚶中分离纯化得到一种均一的多糖组分,具有显著的体外免疫活性。
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Key words
polysaccharide,immunological activity,purification,neutral,Arca subcrenata Lischke,monosaccharide component
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