607 FEMTOSECOND LASER BASED HIGH RESOLUTION OPTICAL COHERENCE TOMOGRAPHY FOR DETECTION OF CYSTITIS CYSTICA IN HUMAN BLADDER - A FEASIBILITY STUDY

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Kidney & Bladder1 Apr 2010607 FEMTOSECOND LASER BASED HIGH RESOLUTION OPTICAL COHERENCE TOMOGRAPHY FOR DETECTION OF CYSTITIS CYSTICA IN HUMAN BLADDER - A FEASIBILITY STUDY Joachim Grosse, Joerg Bornemann, Felix Spöler, Amjad Naami, Ruth Knuchel-Clarke, Gerhard Jakse, and Axel Heidenreich Joachim GrosseJoachim Grosse Murnau, Germany More articles by this author , Joerg BornemannJoerg Bornemann Aachen, Germany More articles by this author , Felix SpölerFelix Spöler Aachen, Germany More articles by this author , Amjad NaamiAmjad Naami Aachen, Germany More articles by this author , Ruth Knuchel-ClarkeRuth Knuchel-Clarke Aachen, Germany More articles by this author , Gerhard JakseGerhard Jakse Aachen, Germany More articles by this author , and Axel HeidenreichAxel Heidenreich Aachen, Germany More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.921AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The feasibility of high resolution optical coherence tomography (HR-OCT) as a non-invasive imaging technique was shown in many cases in the past. With image resolutions down to 1 μm and imaging depths in the range of 1-3 mm this method has now the potential to classify and distinguish the layers in healthy and morphological altered bladder tissue. The objective of our study is to show the feasibility to detect cystitis cystica in the way of virtual histology. METHODS An OCT system comprising a Ti:Sapphire femtosecond laser to support a resolution of 3 μm axial x 7 μm lateral was employed to image 48 fresh human bladder tissue specimens from cystectomies. The OCT tomograms were evaluated double blinded and finally referenced to histological sections using standard HE staining. RESULTS In greyscaled OCT tomograms cystitis cystica appears as a cavernous dark structure surrounded by more scattering brighter tissue. In 10 histological sections of 48 specimens cystitis cystica could be detected by light microscopy (Tab.1). 9 of these 10 specimens were correctly identified by OCT tomograms before (90 %), one sample was categorized as nonexisting urothelium. CONCLUSIONS HR-OCT is a promising modality for the evaluation of bladder microstructures with the possibility to differentiate cystits cystica from normal and cancerous tissue. This might be helpful for the selection of endoscopic bladder biopsies © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e239 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Joachim Grosse Murnau, Germany More articles by this author Joerg Bornemann Aachen, Germany More articles by this author Felix Spöler Aachen, Germany More articles by this author Amjad Naami Aachen, Germany More articles by this author Ruth Knuchel-Clarke Aachen, Germany More articles by this author Gerhard Jakse Aachen, Germany More articles by this author Axel Heidenreich Aachen, Germany More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...