Photopigments of the lateral eye ofLimulus

Photoreceptor membrane fractions of the lateral eye ofLimulus were solubilized in the detergent emulphogene, and three photobleachable materials were observed with respective?max values at 330nm±10nm, 450 nm±10 nm, and 530 nm±10 nm. A530 is the pigment which had been reported earlier by Hubbard and Wald (1960), and it can be separated from A330 and A450 on the basis of differential solubility in digitonin. Approximately the same number of incident quanta were required for a unit absorbance change at?max for all three pigments, but measurable photo-products were not observed after bleaching A330 and A450.