RAPD Analysis between color-different Crab Individuals of Portunus trituberculatus

The blue swimming crabs Portunus trituberculatus with tea-green and/or purple carapace color have been discovered on the shore of eastern part of China these days. In this paper, 40 color-different crab individuals were evaluated using 80 RAPD primers. 30 primers were selected using bulk segregation analysis (BSA) and RAPD-PCR analysis. The results showed that the ratio of polymorphic loci was 61.29%, and Nei's gene diversity was 0.3756, and Shannon's information index was 0.5545 among purple crab individuals; and the ratio of polymorphic loci was 62.44%, and Nei's gene diversity was 0.3451, and Shannon's information index was 0.5099 among tea-green crab individuals. It was clear that the genetic diversity between purple and tea-green crabs was similar, which indicated close phylogenetic relationships between the purple and tea-green crabs. In addition, two primers (SBS A09 and SBS G16) got specific DNA bands by comparing forty individuals of color-different crab individuals through RAPD-PCR analysis and the following electrophoresis. They indicated close phylogenetic relationships between purple and tea-green crabs.