Establishment of Camptothecin Resistant Human Stomach Cancer Cell Line SGC-7901/HCPT and Analysis of Biological Properties

Objective To establish the camptothecin(HCPT) -resistant stomach cancer cell line SGC-7901/HCPT with its characteristics studied. Methods Cell growth curve was painted and the doubling time was accouted by cell counting assay; the camptothecin-resistant stomach cancer cell line SGC-7901/HCPT was established by repeatedly exposing stomach cancer cell line SGC-7901 to increased gradually concentration of HCPT. The resistant index of the resistant cell line to different chemitherapeutic drugs was determined by the methyl thiazolyl tetrazolium(MTT) assay. Cell cycle distribution was measured by flow cytometry(FCM). Results The HCPT-resistant human stomach cancer cell line SGC-7901/HCPT was established after 9 months. The rate of cell proliferation of SGC-7901/HCPT was longer than that of SGC-7901 SGC-7901/HCPT indicated drug-resistance to HCPT,the drug resistant index being 9.583, but were not cross-resistant to Mitomycin C(MMC), Cisplatin(CDDP), 5-fluorouracil(5-Fu),Vincristine sulfate(VCR),Adriamycin(ADM), Daunorubicin(DVR), Etoposide(VP-16). The number of cells in S phrase and G2～M phrase increased(P0.005),suggesting that the DNA cynthesis was increased in SGC-7901/HCPT. Conclusion The camptothecin-resistant stomach cancer cell line SGC-7901/HCPT is established. It can be used for further experiments.