Box I and II motif from myelin basic protein gene promoter binds to nuclear proteins from rodent brain
Journal of molecular neuroscience : MN(1994)
Abstract
The box I and II motif located within the promoter of the myelin basic protein gene contains a simian virus 40 T-antigen-binding
site, a MyoD/E2a-binding site, and a glucocorticoid receptor-binding site. We have found proteins within nuclear extracts
from adult mouse brain, rat embryonic cerebral cortex in culture, and a mouse oligodendrocyte-like cell line that bind to
a32P-labeled synthetic DNA fragment containing the sequences of the box I and II motif. Three major complexes (A, B, and C) were
seen in gel-shift assays. Only complexes A and B were competed out by the unlabeled box I and II fragment or by another synthetic
DNA fragment that also contains sequences similar to a glucocorticoid receptor-binding site. Therefore, complexes A and B
were thought to be specific. The expression pattern of the proteins responsible for the formation of these complexes was also
assessed during development in mouse brain.
MoreTranslated text
Key words
glucocorticoid receptor,dna fragmentation,cell line,binding site
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