HPLC法测定消炎膏中盐酸小檗碱的含量
Nei Mongol Journal of Traditional Chinese Medicine(2011)
Abstract
目的:建立高效液相色谱法测量消炎膏中盐酸小檗碱含量的方法。方法:采用高效液相色谱法,色谱柱为Waters C18(4.6mm×250mm,5μm),流动相为乙腈-0.05mol.L-1磷酸二氢钾(磷酸调pH=3),检测波长为264nm。结果:盐酸小檗碱含量在0.044ug~0.968ug在范围内呈良好线性关系(r=0.9999),平均回收率为98.9%(RSD=1.2%,n=6)。结论:该方法准确、快速、简单,可以作为消炎膏中盐酸小檗碱含量的测量。
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Key words
HPLC,Berberine Hvdrochloridc,XiaoYan Paste
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