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HPLC法测定双丹膏中丹参素的含量

徐亚娟,焦守国

QILU PHARMACEUTICAL AFFAIRS（2009）

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Abstract
目的 采用高效液相色谱法测定双丹膏中丹参素的含量.方法 以十八烷基硅烷键合硅胶为填充剂;以甲醇-1%醋酸溶液(8∶92)为流动相;检测波长为280nm.保留时间大约10min.结果 丹参素的线性范围为14.69～73.44μg (r=0.9999),平均回收率为100.1%(RSD=0.70%,n=6).结论 该方法准确、可靠、重复性好,可作为双丹膏质量控制方法.
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