分光光度法测定葡萄酒中尿素含量
Science and Technology of Food Industry(2012)
Abstract
目的:建立了分光光度法定量检测葡萄酒中尿素含量的方法。方法:采用强阳性离子交换树脂柱对葡萄酒中尿素进行分离,依据费伦反应原理,采用分光光度法测定洗脱液中尿素含量。结果:建立方法的最佳参数为丁二酮单肟最佳添加量6.0g/L,氨基硫脲最佳添加量0.10g/L,反应中煮沸时间为5min,用分光光度计在530nm波长下测定吸光度。方法的最低检出限0.5mg/L,相对标准偏差为3.1%~4.4%,回收率为92.8%~103.1%。结论:此方法简便快速、准确可靠,适合用于葡萄酒样品中尿素含量的定量检测。
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Key words
wine,fearon reagents,urea,spectrophotometric method
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