Effect of Protease Treatment on Inner Quality of Cigar Filler Tobacco

On the foundation of single protease treatments of cigar filler tobacco,an orthogonal experiment was conducted,in which the effects of mixed-protease treatment and other three factors on protein content of cigar filler tobacco and its inner quality were studied.The results indicated that of the single protease treatments,neutral protease and alkaline protease had better effects,with a protein degradation percentage of 18.98% and 17.54%,respectively,and that in the orthogonal experiment,the optimum parameters were: mixed-protease(neutral protease 120 U/g leaf+alkaline protease 4×10-4 AU/g leaf),45 ℃,RH 85%,8 h.With the mixed-protease treatment,cigar filler tobacco protein content decreased by 25.38%,its chemical composition was more balanced and its sensory evaluation quality was improved.