Validated Determination Of Ciproploxacin In Influenza Vaccine By Rp-Hplc

A simple and sensitive high-performance liquid chromatographic method for the determination of ciprofloxacin in influenza vaccine has been developed. The stationary phase was a Purospher RP-18e column (125 x 3.5 mm; 5 mum), the mobile phase consisted of acetonitrile/water/phosphoric acid (85%) (15 : 85 : 0.25, v/v/v %), and its pH was adjusted to pH 3.00 using distilled triethylamine immediately before use. Separation was achieved using a flow rate of 0.6 mL/min at ambient temperature. The ciprofloxacin was detected at 280 nm. The retention time for ciprofloxacin was 4.60 +/- 0.15 min. The limit of detection was 2 ng/mL; the limit of quantification was found to be 5 ng/mL.