New Quantitative Fluorometry For Evaluating Oral Bacterial Adhesion To Biomaterials

This study aimed to develop a simple, quick, and widely-applicable nonradioisotope quantitative method for evaluating adhesion of oral bacteria to dental materials. So, we tried to establish a method using Alamar Blue (R). We used four representative oral bacteria, Streptococcus mummy, Streptococcus sanguinis, Actinomyces naeslundii, and Viellonella atypica. Plastic wells with acrylic resin bottoms were filled with cell suspensions for 2 h. After discarding, the wells were filled with Alamar Blue (R) solution for 3 h, then, the fluorescence intensity of the solution was measured by fluorophotometer. The fluorescence intensity increased with amounts of bacteria in all the species, and the relationship seemed proportional. Alamar blue method is thought to be useful to quantify oral bacterial adhesion to biomaterials.