一株耐锌真菌CTB430-1分离鉴定及其富集特性
Acta Laser Biology Sinica(2013)
Abstract
从某锌尾砂坝土壤中筛选分离、纯化得到一株对锌具有较强抗性的菌株,命名为CTB430-1.对菌株进行形态观察、生理生化试验,采用ITS基因序列分析并构建系统发育树,鉴定该菌为黄曲霉(Aspergillus flavus).研究其对Zn(Ⅱ)抗性和富集特性.结果表明:Zn(Ⅱ)对该菌株的最低抑菌浓度为800 mg/L;菌株对Zn(Ⅱ)的富集量在250 mg/L时达到最大,为40.37 mg/g;采用扫描和透射电镜观察富集前后菌丝体表面形态和内部结构变化,表明Zn(Ⅱ)毒害作用可能导致生长中菌丝表面结构破坏,CTB430-1富集Zn(Ⅱ)由表面吸附和胞内富集两部分组成.
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Key words
phylogenetical,zinc,identification,resistance,fungus,bioaccumulation
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