Ca-dependent regulation of Na+-selective channels via actin cytoskeleton modification in leukemia cells
FEBS Letters(1997)
摘要
With the use of the patch-clamp technique, physiological mechanisms of Na+ channel regulation involving submembranous actin rearrangements were examined in human myeloid leukemia K562 cells. We found that the actin-severing protein gelsolin applied to cytoplasmic surface of membrane fragments at a high level of [Ca2+]i (1 μM) increased drastically the activity of Na-selective channels of 12 pS unitary conductance. In the experiments on intact cells, the elevation of [Ca2+]i using the ionophore 4Br-A23187 also resulted in Na+ channel activation. Addition of actin to the cytoplasmic surface of membrane patches reduced this activity to background level, likely due to actin polymerization. Our data imply that Ca-dependent modulations of the actin cytoskeleton may represent one of the general mechanisms of channel regulation and cell signalling.
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关键词
Na+ channel,Cytoskeleton,Actin,Gelsolin,Ca regulation,Leukemia cell
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