Synthesis Of A New Fluorogenic Substrate For The Continuous Assay Of Mammalian Phosphoinositide-Specific Phospholipase C

The synthesis of a fluorogenic substrate for mammalian phosphoinositide-specific phospholipase C is described. The substrate, based on the widely used fluorescein molecule? is a water-soluble substrate analog of phosphatidylinositol-4-phosphate. The fluorogenic substrate 2 is shown to be a sensitive substrate for human PI-PLC-delta 1 in a continuous assay. (C) 1999 Elsevier Science Ltd. All rights reserved.