HPLC法测定岩黄连中不同部位脱氢卡维丁的含量
Journal of Guangxi Medical University(2010)
Abstract
目的:建立岩黄连中不同部位脱氢卡维丁的高效液相色谱定量测定方法。方法:采用ZorbaxODSC18(250mm×4.6mm,5μm)色谱柱,以乙腈-0.05mol/L磷酸二氢钾溶液(用10%KOH调pH至5.0)(85:15)为流动相,流速1.0mL/min,检测波长为347nm,柱温为室温。结果:脱氢卡维丁在2.51~75.36μg/mL(r=0.99999,n=7)范围内线性关系良好,平均回收率为99.86%,RSD为0.58%。结论:该法简单、快速、重现性好,适用于岩黄连中脱氢卡维丁的定量分析。
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Key words
dehydrocavidine,HPLC,corydalis saxicola bunting,different parts
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