[Effects of 2,2',4,4'-tetrabrominated diphenyl ethers on the TRalpha1 and TRbeta1 expression in liver in C57BL/6 mice].

OBJECTIVE:To study the effect of 2,2',4,4'-tetrabrominated diphenyl ethers (BDE-47) on the expression change of TRalpha1, TRbeta1 at both mRNA and protein levels. METHODS:C57BL/6 mice were divided randomly into 4 groups and administrated with corn oil, 0.5, 5 and 50 mg/kg/BW BDE-47 for 4 days through intraperitoneal injection respectively. GAPDH selected as internal standard, real time quantitative RT-PCR and Western blot were employed to detect the mRNA levels and protein of TRalpha1 and TRbeta1 in livers. RESULTS:Compared with the control group, the mRNA levels of TRalpha1 and TRbeta1 were up-regulated in the medium and high BDE-47 dose groups (P < 0.05). The protein level of TRalpha1 significantly up-regulated while TRbeta1 was significantly down-regulated. CONCLUSION:BDE-47 could change the expression of TRalpha1 and TRbeta1 in the mRNA and protein levels in liver.