HPLC内标法同时测定芪麝丸中9种成分
Chinese Traditional Patent Medicine(2012)
Abstract
目的 建立芪麝丸(黄芪、川芎、青风藤、粉防己等)中9种成分,毛蕊异黄酮、毛蕊异黄酮葡萄糖苷、芒柄花素、芒柄花素苷、洋川芎内酯I、洋川芎内酯A、粉防己碱、防己诺林碱、青藤碱的定量分析方法.方法 采用HPLC内标法,选用异补骨脂素为内标物,样品经过甲醇提取,采用Kromasil C18柱,甲醇-0.2%甲酸水溶液为流动相,梯度洗脱,紫外检测波长262nm.结果 毛蕊异黄酮、毛蕊异黄酮葡萄糖苷、芒柄花素、芒柄花素苷、洋川芎内酯I、洋川芎内酯A、粉防己碱、防己诺林碱、青藤碱分别在13.8~440μg/mL、25.9~830μg/mL、9.7~310μg/mL、6.6~212μg/mL、6.3~200μg/mL、6.3~200μg/mL、14.1~450.μg/mL、11.3~360μg/mL、50.9~1630μg/mL范围内呈良好线性关系,内标法与标准曲线法测定结果一致.结论 该方法准确可靠,可用于芪麝丸的质量控制.
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Key words
sinomenine,calycosin-7-glucoside,HPLC internal standard method,calycosin,senkyunolide I,formononetin,ononin,Qishe Pill,fangchinoline,tetrandrine,senkyunolide A
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