High Performance Chromatography of Amino Acids, Peptides and Proteins XXIII · Peptide Mapping by Hydrophilic Ion-paired, Reversed-Phase High Performance Liquid Chromatography for the Characterisation of the Tryptic Digest of Haemoglobin Variants

Abstract The application of high performance liquid chromatography on reversed-phase columns for the tryptic mapping of haemoglobin variants is reported. The effect of flow rate and gradient shape on resolution and column efficiencies has been examined using acetonitrile-water-orthophosphoric acid elution systems. With these conditions it is possible to recognise sequence changes for variant haemoglobins including HbC, HbS, HbE, HbJ Cambridge and HbD Punjab.