周期性机械牵张对大鼠肺泡巨噬细胞TLR4表达的影响
Journal of Shandong University(Health Sciences)(2013)
Abstract
目的 探讨周期性机械牵张对大鼠肺泡巨噬细胞(AM) Toll样受体4(TLR4)表达的影响.方法 采用美国Flexercell公司生产的Flexercell 4000TTM应力加载系统,对体外培养的AM进行周期性牵张.实验分为机械牵张6h组、8h组(30%应变率,0.3Hz频率,方波)和静止对照组.RT-PCR检测AM上TLR4 mRNA的表达;ELISA检测巨噬细胞炎性蛋白2(MIP-2)的浓度;免疫细胞化学法检测AM上TLR4蛋白表达.结果 与静止对照组比较,机械牵张6h组、8h组TLR4 mRNA、MIP-2蛋白及TLR4蛋白表达显著升高(P<0.01).结论 机械牵张导致了大鼠肺泡巨噬细胞TLR4和MIP-2表达上调.
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Key words
Alveolar macrophages,Lung injury,Immunocytochemistry,RT-PCR,Toll-like receptor 4,Cyclic mechanical stretch
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