Screen of phospholipase A1 production strain.

Phospholipase A1 is the single-minded enzyme which can hydrolyze natural phosphatide Sn-1 position acyl to get Sn-2 acyl lyso-phospholilid and fatty acid. The phospholipase may use in the trade in the refinery process (oil degum) to remove phospholipids. The strain named PL-06 which has high phospholipase A1 is obtained from the rich oil soil by using lecithin as the only carbon source and it's fermentation conditions of shake flask is optimized in this paper. The result showed that the best nitrogen source was NH4Cl and the best complex carbon source was the combination of malt sugar and lecithin, the density respectively is 2.0% and 0.5%, and the best salt is FeSO4. In such condition, the activity of phospholipase A1 of PL-06 can reach 18.68U/mL which is 1.73 times higher than that of the non- optimized condition( 6.83U/mL ) after 48 hours fermentation.