Antral Nodular Gastritis is Strongly Associated With Diffuse-Type Gastric Cancer and Useful for Identifying High-Risk Groups Not Detected by Serum Pepsinogen Tests

colonized bacteria in stomach was estimated as the copy number of 16S rRNA gene by the real-time PCR analysis using the H. heilmannii-specific primer pair of 16S rRNA gene.Results: Gastric MALT lymphoma formation by infection with H. heilmannii was observed in C57BL/ 6J mice, but it was not detected in IL-10 knockout mice.In the IL-10 knockout mice, a large number of infected bacteria were observed as much as the same amounts of C57BL/6J mice.Microarray and KEGG pathway analyses revealed that cell migration related pathways, namely the Intestinal immune network for IgA production (hsa04672) and the Leukocyte transendothelial migration (hsa04670), were activated in the H. heilmannii TKY infected C57BL/6J mice.Quantitative RT-PCR analysis confirmed that the expression of several integrin genes (α4, αL, αM, β2, and β7) and TNF-receptor super family genes (BCMA, TACI, and BAFFR), were significantly upregulated by infection with H. heilmannii in C57BL/ 6 mice.However, these induced gene expressions were not shown in IL-10 knockout mice.Conclusion: Cell migration related pathway cascade is activated in the H. heilmannii TKY infected gastric mucosa through IL-10 dependent signaling.These might be the cause of gastric MALT lymphoma by infection with H. heilmanii.