稀释方法对绒山羊冷冻精液品质的影响
China Animal Husbandry & Veterinary Medicine(2009)
Abstract
选取7只精液品质优良的雁门绒山羊种公羊,通过测定其冷冻精液解冻后的精子活率(0、4 h)、精子存活指数、顶体完整率及精清中透明质酸酶(HYD)、谷草转氨酶(GOT)、乳酸脱氢酶(LDH)酶活力,研究一步稀释法和二步稀释法对冷冻精液品质的影响。结果表明,二步稀释法解冻后的精子存活指数显著(P<0.05)高于一步稀释法,其解冻后精清中透明质酸酶、谷草转氨酶、乳酸脱氢酶的活力均显著(P<0.05)低于一步稀释法。表明采用二步稀释法冷冻的绒山羊精液品质好于一步稀释法。
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Key words
frozen semen,dilution method,cashmere goats
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