香菇多糖径向流色谱分离工艺条件优化

Forest By-Product and Speciality in China(2011)

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Abstract
在单因素试验的基础上,通过Box-Benhnken组合设计和响应曲面分析法优化香菇多糖径向流色谱分离的工艺条件。响应面分析结果表明,当样品浓度为10mg/mL,上样量31mL,上样流速19mL/min,洗脱流速27mL/min,在次条件下多糖回收率达到87.33%,比传统轴向色谱分离及化学方法效率更高,时间更短。
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Key words
RSM,Separation,Radial flowchromatography,Lentinula edodes polysaccharides
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