1H NMR studies of the Fc region of human IgG1 and IgG3 immunoglobulins: Assignment of histidine resonances in the CH3 domain and identification of IgG3 protein carrying G3m(st) allotypes

A 1H NMR study of the Fc region of human IgG1 and IgG3 immunoglobulins is presented. 1H NMR data were collected for the Fc and pFc' fragments obtained from human monoclonal IgG1 and IgG3 and also from rabbit IgG. The C2-H proton signal of His-435 in the CH3 domain of IgG1 was assigned by comparing the spectra of the Fc fragment of IgG1 with that of IgG3[G3m(g)] where there is a substitution of histidine by arginine at position 435. Chemical shifts and linewidths of the His-435 signal are quite different for the Fc and pFc' fragments. We suggest that His-435 is involved in the interdomain CH2-CH3 contact. Assignments of the C2-H proton signals of His-429 and His-433 in the CH3 domain were made on the basis of our previous 1H NMR results on the human light chain.