Preparation Of Arsenic Trioxide-Loaded Albuminutes Immuno-Nanospheres And Its Specific Killing Effect On Bladder Cancer Cell In Vitro

Background Recently, arsenic trioxide (As, 0,) was considered as a novel anti-tumor agent. However, it showed severe toxicity effect on normal tissue at the same time. To improve its therapeutic efficacy and decrease its toxicity, we prepared arsenic trioxide-loaded albuminutes immuno-nanospheres [As2O3-( HAS-NS) -BDI-1] targeted with nonoclonal antibody ( McAb) BDI-1 and tested its specific killing effect against bladder cancer cell.Methods As2O3-HAS-NS was prepared by chemical cross-linking method. Monoclonal antibody BDI-1 was purified with ammonium sulphate saltingout and chromatography. Albuminutes microspheres were conjugated with McAb by SPDP cross-linking method. Concentration of As in As2O3- (HAS-NS)-BDI-1 and As2O3-HAS-NS was measured by atomic fluometry method. As2O3-(HAS-NS) -BDI-1 and its activity were detected by SDS-PAGE reduction electrophoresis, indirect immunofluorescence test, light microscope and scanning electron microscope observation. Acridine orange staining and tritiated thymidine (H-3-TdR) incorporation tests were used to indicate specific killing activity of As2O3-(HAS-NS) -BDI-1 in vitro.Results In As2O3-(HAS-NS)-BDI-1 groups, we saw two protein bands in SDS-PAGE reduction electrophoresis. Albuminutes immuno-nanospheres were rounded with clear green fluorescence by immunofluorescence test. Under microscope, we observed that BIU-87 cells were covered with the As2O3-(HAS-NS)-BDI-1 and that As2O3-(HAS-NS)-BDI-1 moved with the BIU-87 cells. The albuminutes immuno-nanospheres were tightly junctioned with the BIU-87 cells. Specific killing activity of As2O3-(HAS-NS) -BDI-1 on bladder tumor cells was observed by acridine orange staining and H-3-TdR incorporation assays.Conclusions As2O3-(HAS-NS)-BDI-1 might bind specifically against BIU-87 cells, thus leading to high activity of killing bladder tumor cells.