Protection Of Mice With Vaccinia Virus Recombinants That Express The Rabies Nucleoprotein
VIROLOGY(1991)
摘要
The role of rabies virus nucleoprotein (N) in protection against rabies was examined with recombinant vaccinia viruses expressing the N of the Challenge Virus Standard strain. Two chimeric plasmids were constructed with the open reading frame of the N gene placed downstream of the vaccinia P 7.5 promoter (early/late class) or the vaccinia P 11 promoter (late class), with each expression cassette flanked by vaccinia thymidine kinase (TK) sequences to enable marker rescue by TK insertional inactivation. Two recombinants were isolated that expressed the rabies N in infected cells as determined by radioimmunoprecipitation and immunofluoresence microscopy with an anti-N monoclonal antibody. Two groups of 25 ICR mice inoculated intradermally with the recombinants and challenged with 75 MFPLD 50 of street rabies virus showed high survival ratios ( 22 25 and 21 25 ). Intramuscular inoculation, however, was not protective against 25 MFPLD 50 . The intradermally vaccinated mice developed non-neutralizing antibodies against rabies N.
更多查看译文
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要