Subsite structure of maltogenic amylase from Thermomonospora viridis TF-35.

We estimated the subsite structure of the maltogenic amylase from Thermomonospora viridis TF-35 (TVA). TVA has six subsites, and the catalytic site is between the 3rd and 4th subsite. The subsite affinities, A (- 3), A (- 2), (A (- 1) + A (+ 1)), A (+ 2) and A (+ 3) were calculated to be 0.47, 2.31, 0.49, 2.45, and 0.17 kcal mol (- 1), respectively.