ATP生物发光法快速测定生乳中细菌总数的研究
Chinese Journal of Health Laboratory Technology(2009)
Abstract
目的:探索利用ATP生物发光技术测定生乳中细菌量的可行性。方法:利用生物发光技术测定细菌中ATP量,根据细菌中ATP量与细菌数成正比这一原理,推算出生乳中菌落总数,与国标平皿计数法进行对比,分析两者之间的相关性。结果:检测结果与传统国标法有很好的相关性(r2=0.9334,y=0.6901x+0.6505)。结论:ATP生物发光技术具有准确、便利等优点,可用于生乳中细菌的快速检测。
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Key words
Counting of bacterium,Crude milk,Bioluminescence technique
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