地态离体培养植株再生及其栽培试验

Acta Horticulturae Sinica(2007)

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Abstract
地菍叶片离体培养研究结果表明:诱导愈伤组织最佳的培养基为MS+1 mg·L-1 2,4-D+0.2mg·L-1 6-BA+5%蔗糖+0.6%琼脂和MS+2 mg·L-1 2,4-D+0.2 mg·L-1 6-BA+5%蔗糖+0.6%琼脂;丛生芽分化增殖最佳培养基为Ms+2 mg·L-1 6-BA+0.3 mg·L-1 NAA+5%蔗糖+0.6%琼脂;生根最佳培养基为1/2MS+1.5 mg.L-1 NAA+0.5 mg·L-1 2,4-D+5%蔗糖+0.6%琼脂和MS+0.5 mg·L-1 6-BA+2 mg·L-1 NAA+5%蔗糖+0.6%琼脂.栽培观赏研究表明:盆栽最佳温度为28~32℃;最佳基质为泥炭土+蛭石(2:1)和腐殖土+蛭石(2:1);坪栽最佳肥料配比为N:P:K=1.4:1.2:1,最佳土壤为腐殖土.
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Key words
Regeneration,Melastoma dodecandrum,In vitro culture,Ornamental,Cultivation
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