背角无齿蚌和三角帆蚌的rDNA基因ITS1及ITS2序列的初步研究
CHINESE JOURNAL OF FISHERIES(2010)
Abstract
对背角无齿蚌(Anodonta woodiana woodiana)和三角帆蚌(Hyriposis cumlngii)的核糖体RNA内转录间隔区1(ITS1)和内转录间隔区2(ITS2)进行扩增测序.结果显示,背角无齿蚌(A.woodiana woodiana)的ITS1长度为496bp(A:20.36%;T:25.40%;G:27.42%;C:26.82%);ITS2长度为466bp(A:24.89%;T:21.89%;G:26.39%;C:28.83%);三角帆蚌(H. cumingii)的ITS1为475bp(A:21.47%;T:23.79%;G:29.36%;C:25.48%);ITS2为519bp(A:25.82%;T:22.93%;G:25.24%;C:26.01%).研究表明ITS在不同科的物种间差异较大,不适合用于高阶元的系统发育研究.背角无齿蚌、三角帆蚌、魁蚶、栉孔扇贝、大珠母贝5个物种的ITS2中有一段保守序列,推测可能与ITS的某一功能有关.
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Key words
anodonta woodiana woodiana,rdna,its2 sequences
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