Isolation and identification of activated substances of North Ph. praecox leaves

Objective:To extract and identify activated substances from North Ph. praecox leaves. Methods:The activated substances of North Ph. praecox leaves were extracted by ethanol-aqueous solution with ultrasonic-assisted extraction,and the extracts were further purified and isolated by SP825 resin column chromatograph. C-glycosides and phenolic acids in the extracts were analyzed qualitatively,and the contents of C-glycosides and phenolic acids in different eluent were quantified by detective high-performance liquid chromatography (HPLC). Results:C-glycosides and phenolic acids were detected in crude extracts,and the proportion of C-glycosides is 0.84%,phenolic acids is 0.31%. After purifying by SP825 resin column chromatograph,the content of C-glycosides in 30% ethanol eluent was 12.8%,one of phenolic acids in 10% ethanol eluent was 5%. Conclusion:Ethanol-aqueous solution with ultrasonic-assisted extraction can extract activated substances from North Ph. praecox leaves effectively,and SP825 resin column chromatograph can further isolate different activated substances.