反相高效液相色谱分析牡丹皮中丹皮酚含量变化

Journal of Heze Medical College(2011)

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Abstract
目的利用反相高效液相色谱法(RP-PLC)对菏泽产地牡丹皮中丹皮酚含量进行分析。方法色谱条件C10色谱柱;丹皮酚测定所用流动相为甲醇-磷酸(60∶40),检测波长为274 nm。结果丹皮酚的线性范围在0.10~0.50μg,平均回收率98.06%,RSD=1.68%。结论所选牡丹皮的丹皮酚含量为1.62%。本方法简便、快捷、重现性好,适用于丹皮酚的含量测定,可作为其质量控制方法。菏泽牡丹皮中丹皮酚含量较高,有较好的药用价值。
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Key words
RP-HPLC,determination,paeonol
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