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烟草WS38生长素结合蛋白基因cDNA的克隆及序列分析

刘晓柱,黄妤,彭珍子,周晶辉,洪亚辉,张学文

JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY(NATURAL SCIENCES)（2010）

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Abstract
以烟草WS38为材料,根据GenBank中登录的烟草生长素结合蛋白(ABP1)氨基酸序列设计引物,通过RT-PCR克隆了烟草WS38 ABP1基因cDNA分子编码区,序列全长为564 bp,可编码1条188个氨基酸的多肽.利用Clustal X软件对该序列翻译获得的多肽序列与报道的烟草生长素结合蛋白氨基酸序列比较,两者同源性为98.4%,存在2个氨基酸的差异,但差异氨基酸对ABP1结构和功能影响微弱.认为克隆的cDNA序列即为烟草WS38 ABP1 cDNA,不同品种烟草ABP1基因存在的核苷酸单位点多态性(SNP)造成了两者氨基酸序列的差异.
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